Invention Grant
- Patent Title: End modification to prevent over-representation of fragments
- Patent Title (中): 结束修改以防止片段的过度表示
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Application No.: US12223761Application Date: 2007-02-07
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Publication No.: US09012184B2Publication Date: 2015-04-21
- Inventor: Roberto Rigatti , Niall Anthony Gormley , Helen Rachel Bignell
- Applicant: Roberto Rigatti , Niall Anthony Gormley , Helen Rachel Bignell
- Applicant Address: GB Nr Saffron Walden
- Assignee: Illumina Cambridge Limited
- Current Assignee: Illumina Cambridge Limited
- Current Assignee Address: GB Nr Saffron Walden
- Agency: Illumina, Inc.
- Agent Brent C. Moore
- International Application: PCT/GB2007/000427 WO 20070207
- International Announcement: WO2007/091064 WO 20070816
- Main IPC: C12Q1/68
- IPC: C12Q1/68 ; C12N15/10

Abstract:
The invention relates to a method of preparing a 5′ and 3′ modified library of template polynucleotides and also the use of the 5′ and 3′ modified library of templates in methods of solid-phase nucleic acid amplification. In particular, the invention relates to a method of preparing a 5′ and 3′ modified library of template polynucleotides which have common sequences at their 5′ ends and at their 3′ ends, wherein over-representation of “end” sequences of the primary polynucleotide molecules from when the 5′ and 3′ modified library is generated is greatly reduced or prevented.
Public/Granted literature
- US20090176662A1 End Modification to Prevent Over-Representation of Fragments Public/Granted day:2009-07-09
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