Invention Grant
- Patent Title: Methods for identifying leukemia stem cells and distinguishing them from normal hematopietic stem cells in patients with acute myeloid leukemia: uses in diagnosis, treatment, and research
- Patent Title (中): 识别白血病干细胞并与急性骨髓性白血病患者正常造血干细胞区别的方法：诊断，治疗和研究中的应用
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Application No.: US13625369Application Date: 2012-09-24
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Publication No.: US09012215B2Publication Date: 2015-04-21
- Inventor: Jonathan Michael Gerber , Richard John Jones
- Applicant: Jonathan Michael Gerber , Richard John Jones
- Applicant Address: US MD Baltimore
- Assignee: The Johns Hopkins University
- Current Assignee: The Johns Hopkins University
- Current Assignee Address: US MD Baltimore
- Agency: Johns Hopkins Technology Transfer
- Main IPC: C12N5/095
- IPC: C12N5/095 ; C12N5/09 ; G01N33/574 ; C12Q1/32
Abstract:
Using the methods of the present invention, intermediate (int) levels of aldehyde dehydrogenase (ALDH) activity reliably distinguished leukemic CD34+CD38− cells capable of engrafting immunodeficient mice, from residual normal hematopoietic stem cells that exhibited relatively higher ALDH activity. Minimal residual disease (MRD) detected during complete remission was enriched for the CD34+CD38−ALDHint leukemic cells, and the presence of these cells after therapy highly correlated with subsequent clinical relapse. The methods of the present invention can distinguish normal from leukemic CD34+CD38− cells, and identifies those AML cells associated with relapse. Methods of prediction of relapse of AML patients and methods of treatment are also provided.
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