Invention Grant
US09023615B2 Gram staining method with improved decolorization of the crystal violet-iodine complex from gram negative bacteria
有权
革兰氏染色法，改善革兰氏阴性细菌结晶紫 - 碘络合物的脱色
- Patent Title: Gram staining method with improved decolorization of the crystal violet-iodine complex from gram negative bacteria
- Patent Title (中): 革兰氏染色法，改善革兰氏阴性细菌结晶紫 - 碘络合物的脱色
-
Application No.: US13773864Application Date: 2013-02-22
-
Publication No.: US09023615B2Publication Date: 2015-05-05
- Inventor: Charles Weidner , Cynthia Connolly , Lesley Schumack , Parula Mehta , Setareh Duquette
- Applicant: Ventana Medical Systems, Inc.
- Applicant Address: US AZ Tucson
- Assignee: Ventana Medical Systems, Inc.
- Current Assignee: Ventana Medical Systems, Inc.
- Current Assignee Address: US AZ Tucson
- Agency: Klarquist Sparkman, LLP
- Main IPC: G01N1/30
- IPC: G01N1/30 ; G01N33/48 ; C12Q1/00 ; C12M1/00 ; C12M3/00 ; G01N1/31 ; C12Q1/04
Abstract:
Provided herein are methods of staining biological material for the purpose of detecting, and in some examples also identifying, microorganisms. Methods of Gram staining bacteria using a slow-acting decolorizing formulation, such as one that includes 1,2-propandiol or ethylene glycol, can be used to extend the time of the decolorizing step, and thus permit automation of the Gram staining method. Also provided are compositions and kits for performing automated Gram staining on microscope slides.
Public/Granted literature
Information query
