Invention Grant
US09023637B2 Enhanced citric acid production in Aspergillus with inactivated asparagine-linked glycosylation protein 3 (Alg3), and/or increased LaeA expression
有权
增强柠檬酸生产曲霉与灭活天冬酰胺连接的糖基化蛋白3（Alg3）和/或增加的LaeA表达
- Patent Title: Enhanced citric acid production in Aspergillus with inactivated asparagine-linked glycosylation protein 3 (Alg3), and/or increased LaeA expression
- Patent Title (中): 增强柠檬酸生产曲霉与灭活天冬酰胺连接的糖基化蛋白3（Alg3）和/或增加的LaeA表达
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Application No.: US13691396Application Date: 2012-11-30
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Publication No.: US09023637B2Publication Date: 2015-05-05
- Inventor: Ziyu Dai , Scott E. Baker
- Applicant: Battelle Memorial Institute
- Applicant Address: US WA Richland
- Assignee: Battelle Memorial Institute
- Current Assignee: Battelle Memorial Institute
- Current Assignee Address: US WA Richland
- Agency: Klarquist Sparkman, LLP
- Main IPC: C12N1/15
- IPC: C12N1/15 ; C12P7/48 ; C12N1/14 ; C12N9/10 ; C12N15/00 ; C12P21/00 ; C07H21/00
Abstract:
Provided herein are fungi, such as Aspergillus niger, having a dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichyl mannosyltransferase (Alg3) gene genetic inactivation, increased expression of a loss of aflR expression A (Lae), or both. In some examples, such mutants have several phenotypes, including an increased production of citric acid relative to the parental strain. Methods of using the disclosed fungi to make citric acid are also provided, as are compositions and kits including the disclosed fungi.
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