An analysis method includes spectroscopically separating light from a light source via a subject into plural wavelength ranges, imaging the subject with respect to each wavelength range, and thereby, acquiring plural spectroscopic images, dividing a subject image into plural areas in each of the spectroscopic images, analyzing a spectrum of the spectroscopically-separated lights of each area with respect to the plural spectroscopic images, and thereby, analyzing a spectral characteristic, and analyzing a component of the subject based on the spectral characteristic in at least one area of the plural areas, and has a pixel selection step of eliminating the area having the same spectral characteristic as the spectral characteristic with respect to the light from the light source from objects of the analysis of the component before the analyzing of the component.