A photolithography alignment mark and a mask and semiconductor wafer containing said mark are described. The alignment mark comprises: a plurality of first alignment lines arranged parallel with each other in a first direction; a plurality of second alignment lines arranged parallel with each other in a second direction perpendicular to the first direction, and wherein each of the plurality of first alignment lines is composed of a predetermined number of first fine alignment lines uniformly spaced from each other, and each of the plurality of second alignment lines is composed of a predetermined number of second fine alignment lines uniformly spaced from each other. Alignment marks can be located in non-circuit pattern regions of the mask and on a plurality of layers in mark regions on the wafer.