Disclosed is a method for generating and transmitting a reference signal in a clustered DFT-spread OFDM transmission scheme. A method for generating and transmitting a DM-RS in a clustered DFT-spread-OFDM scheme comprises: a step of generating DM-RS sequences corresponding to the number of clusters allocated for an uplink transmission; and a step of mapping the generated DM-RS sequences to the relevant DM-RS symbol positions for each cluster. Accordingly, the method for generating and transmitting a reference signal according to the present invention, in which DM-RS sequences are allocated and transmitted on a cluster basis, uses a complete DM-RS sequence for each cluster, and therefore inter-cell interference can be weakened, and problems which might occur when applied to a multi-user MIMO (MU-MIMO) scheme can be solved.