A cartridge for holding and dispensing streaking applicators for an automated streaking apparatus having a cartridge holder, the cartridge including: a case with a dispensing end, a longitudinal axis and an elongate slot generally parallel to the longitudinal axis, the dispensing end including an applicator dispensing position where one applicator can be at least partially accessible from outside the case; and an applicator support member constrained within the case for longitudinal movement therein and being able to carry a stack of applicators; the elongate slot being capable of operatively receiving a biasing member when the cartridge is in the cartridge holder to urge the support member towards the dispensing end and an applicator into the dispensing position.