Methods, systems, and computer readable media for utilizing a plurality of pre-encoded payloads in a stream transmission are disclosed. According to one aspect, a method includes receiving a plurality of payload streams associated with an audio stream clip file and selecting a first payload stream encoded at a first bit rate from the plurality of payload streams as the payload for a packet stream transmission being sent to a device under test. The method also includes receiving a bit rate change request that indicates a second payload stream encoded at a second bit rate is to be used as the payload for the packet stream transmission and conducting a synchronization procedure that synchronizes a first packet of the second payload stream with a last packet of the first payload stream in order to transition the packet stream transmission from the first bit rate to the second bit rate.