A cleaning structure for floating oil and substance on water surface provided by the present invention comprises at least one cleaning ball and a threading rope. The cleaning ball comprises a plurality of yarns arranged in parallel, and a tie is used for bundling the yarns at the middle section to form a ball structure. A fixing ring is threaded with the tie, and the threading rope is threaded through the fixing ring of each of the cleaning balls. The tie of each of the cleaning balls can be threaded with the threading rope in equal intervals beforehand, and the cleaning balls formed by bundling the side by side arranged yarns with the ties are arranged on the threading rope in equal intervals to form the cleaning structure. The yarns of the cleaning ball can be made of water repellent fiber yarn or water repellent reticulated yarn which can be spread as an expanded absorbing surface while floating on water surface, and the gaps of each of the fiber yarns are formed as spaces for absorbing floating oil and substance. The cleaning structure made of the water repellent material can be prevented from absorbing water and sinking. Thereby, floating oil and substance can be cleaned effectively.