Embodiments include systems and methods for accurately controlling gain of a high-speed variable-gain amplifier (VGA) without adversely impacting bandwidth performance. Embodiments include a VGA with a variable resistor, for which resistance is a function of a control level. A gain calibration system controls the control level by using a gain control feedback subsystem to sample outputs of a duplicate VGA, which includes a duplicate variable resistor. The sampled duplicate outputs are compared to a target gain generated by a reference generator. The control level can be fed back to control the gain of the duplicate VGA until the target gain is reached. The control level can also be fed to the actual VGA to control its gain. By performing gain control on the duplicate VGA without interfering with the output signal path of the actual VGA, the actual VGA's gain can be accurately controlled without impacting its bandwidth.