A puncture instrument has a simple configuration and can accurately control the insertion position and direction of the puncture instrument when in use to form a pilot hole in the vertebral pedicle. The puncture instrument is configured by including a puncture needle 2 for forming the pilot hole, and a reference bar 3 that is provided at the puncture needle to have a predetermined angle with respect to the puncture needle 2 and serves as a reference for guiding the puncture needle 2 to a desired position at a desired angle. With the present invention, the puncture needle 2 is guided by the reference bar 3 to a desired position at a desired angle, and hence the puncture needle can be controlled by a simple configuration as compared with the case where a guiding device is separately provided.