A battery assembly provided with an adhesive stop mechanism is disclosed. The battery assembly includes multiple battery cells, a primary retaining frame, a secondary retaining frame, two common electrodes and a bonding layer. The primary and second retaining frames are combined together to constitute accommodation chambers for housing the battery cells. The primary retaining frame includes an outer deck and a shallow deck, wherein the outer deck is formed with adhesive application pores and the shallow deck is formed with stop portions corresponding to the adhesive application pores. The adhesive composition applied through the adhesive application pores is confined by the stop portions and subsequently cured into a bonding layer that firmly holds the battery cells within the accommodation chambers.