Invention Grant
US09045595B2 Process for recovering polyhydroxialkanoates (“PHAs”) from cellular biomass
有权
从细胞生物质中回收多羟基亚油酸酯(“PHAs”)的方法
- Patent Title: Process for recovering polyhydroxialkanoates (“PHAs”) from cellular biomass
- Patent Title (中): 从细胞生物质中回收多羟基亚油酸酯(“PHAs”)的方法
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Application No.: US10596077Application Date: 2004-11-25
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Publication No.: US09045595B2Publication Date: 2015-06-02
- Inventor: Paulo Eduardo Mantelatto , Alvaro Minto Duzzi , Tetuhiko Sato , Nazareno Antonio Sertori Durao , Roberto Vianna Nonato , Carlo Rocchiccioli , Sonia Maria Kesserlingh
- Applicant: Paulo Eduardo Mantelatto , Alvaro Minto Duzzi , Tetuhiko Sato , Nazareno Antonio Sertori Durao , Roberto Vianna Nonato , Carlo Rocchiccioli , Sonia Maria Kesserlingh
- Applicant Address: BR Sao Paulo
- Assignee: PHB Industrial S.A.
- Current Assignee: PHB Industrial S.A.
- Current Assignee Address: BR Sao Paulo
- Agency: Fish & Richardson P.C.
- Priority: BR0306230-9 20031128; BR0405622-1 20041119
- International Application: PCT/BR2004/000237 WO 20041125
- International Announcement: WO2005/052175 WO 20050609
- Main IPC: C08G63/90
- IPC: C08G63/90 ; C08G63/89 ; C12P7/62 ; C08G63/06

Abstract:
A process for recovering polyhydroxyalkanoates (PHAs) from cellular biomass of bacteria, said biomass being obtained by fermentation and in the form of a cellular biomass slurry in aqueous suspension, comprising the steps of: submitting the slurry to operations of injection of PHA solvent, agitation and heating, in order to form a suspension comprising PHA solvent with the dissolved PHA, water and insoluble residues; recuperating the solvent enriched with PHA; rapidly cooling the solution of PHA solvent to precipitate the dissolved PHA; micro-filtrating the suspension of PHA precipitated in the solvent, in order to separate a paste concentrated with precipitated PHA; washing with water, heating and agitating the concentrated PHA paste, to promote evaporation of the solvent and to obtain a suspension containing PHA granules; agitating and shearing the PHA granules and depleting the residual solvent; and separating the purified PHA particles from the suspension.
Public/Granted literature
- US20070161096A1 Process for recovering polyhydroxialkanoates ("phas") from cellular biomass Public/Granted day:2007-07-12
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