A grapevine cultivar designated JB05-22-3-27 is disclosed. The invention relates to the plants of grapevine cultivar JB05-22-3-27, to the plant parts of grapevine cultivar JB05-22-3-27 and to methods for producing a grapevine plant produced by crossing grapevine cultivar JB05-22-3-27 with itself or with another grapevine variety. The invention also relates to methods for producing a grapevine plant containing in its genetic material one or more transgenes and to the transgenic grapevine plants and plant parts produced by those methods. This invention also relates to grapevine cultivars or breeding cultivars and plant parts derived from grapevine variety JB05-22-3-27, to methods for producing other grapevine cultivars, lines or plant parts derived from grapevine cultivar JB05-22-3-27 and to the grapevine plants, varieties, and their parts derived from use of those methods. The invention further relates to hybrid grapevine fruits, seeds, plants and plant parts produced by crossing grapevine cultivar JB05-22-3-27 with another grapevine cultivar.