Invention Grant
- Patent Title: Method for production of euglena containing wax ester at high content, and method for production of wax ester
- Patent Title (中): 高含量含有赤藓珠蜡的生产方法以及蜡酯的制造方法
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Application No.: US13811120Application Date: 2011-07-13
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Publication No.: US09045784B2Publication Date: 2015-06-02
- Inventor: Ryo Arashida , Sharbanee Mitra
- Applicant: Ryo Arashida , Sharbanee Mitra
- Applicant Address: JP Tokyo
- Assignee: Euglena Co., Ltd.
- Current Assignee: Euglena Co., Ltd.
- Current Assignee Address: JP Tokyo
- Agency: Sughrue Mion, PLLC
- Priority: JP2010-163370 20100720
- International Application: PCT/JP2011/066015 WO 20110713
- International Announcement: WO2012/011421 WO 20120126
- Main IPC: C12P7/64
- IPC: C12P7/64 ; C12N1/12 ; C12N15/82
Abstract:
Provided is a method for producing a euglena highly containing a wax ester, comprising aerobically culturing a microalga euglena by photosynthesis using carbon dioxide as a carbon source, then further culturing the microalga euglena under nitrogen-starvation conditions to increase paramylon accumulation per cell, and subsequently putting the microalga euglena under anaerobic conditions, so that a euglena highly containing a wax ester can be produced; and a method for producing a wax ester.The present invention relates to a method for producing a euglena highly containing a wax ester. The method comprises a first step of aerobically culturing a microalga euglena, a second step of further culturing a medium under nitrogen-starvation conditions, and a third step of retaining the cells under anaerobic conditions.
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