A driving method of pixels of a display panel is provided, which is adapted to drive a pixel of the display panel corresponding to a target grey-level of a display frame. In this method, a first pixel voltage is applied to the pixel during a first display period. At least one second pixel voltage is applied to the pixel during a second display period. The first and second display periods correspond to the display frame, and an enabling duration of the second display period is longer than the enabling duration of the first display period. When the target grey-level is not equal to a limit grey-level, the grey-level corresponding to the pixel voltage is different from the grey-level corresponding to the second pixel voltages. A sum of optical effects of the pixel during the first and second display periods is equal to the optical effect of the target grey-level.