Example embodiments presented herein are directed towards the elimination of physical layer identity ambiguities for uplink detection in a wireless network. Some of the example embodiments are directed towards a source network node, and corresponding methods therein, for assigning an unique uplink physical layer identity for a user equipment uplink transmission of a first user equipment being served by the source network node. The unique uplink physical layer identity is provided to ensure that at least one second user equipment, which may be in proximity to a non-serving cell associated with the uplink transmission, does not have a physical layer identity ambiguity with the first user equipment. Some of the example embodiments are also directed towards a target network node, and corresponding methods therein, for performing uplink detection of the first user equipment using the unique physical layer identity described above.