Invention Grant
US09050362B2 Hybrid superparamagnetic iron oxide nanoparticles and polyethylenimine as a magnetocomplex for gene transfection
有权
混合超顺磁性氧化铁纳米颗粒和聚乙烯亚胺作为基因转染的磁复合物
- Patent Title: Hybrid superparamagnetic iron oxide nanoparticles and polyethylenimine as a magnetocomplex for gene transfection
- Patent Title (中): 混合超顺磁性氧化铁纳米颗粒和聚乙烯亚胺作为基因转染的磁复合物
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Application No.: US13848917Application Date: 2013-03-22
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Publication No.: US09050362B2Publication Date: 2015-06-09
- Inventor: Li-Fang Wang , Shuo-Li Sun , Yu-Lun Lo
- Applicant: Kaohsiung Medical University
- Applicant Address: TW Kaohsiung
- Assignee: Kaohsiung Medical University
- Current Assignee: Kaohsiung Medical University
- Current Assignee Address: TW Kaohsiung
- Agency: Alston & Bird LLP
- Priority: TW99144853A 20101220
- Main IPC: A61K9/50
- IPC: A61K9/50 ; A61K48/00 ; B82Y5/00 ; C08K9/04 ; C08K9/08 ; B82Y30/00 ; C12N15/85
Abstract:
Disclosed are the nanoparticle and the method for the same, and the preparing method includes steps of mixing polyethylenimine (PEI) with the poly(acrylic acid)-bound iron oxide (PAAIO) to form a PEI-PAAIO polyelectrolyte complex (PEC) and mixing the PEI-PAAIO PEC with genetic material such as plasmid DNA to form the PEI-PAAIO/pDNA magnetic nanoparticle. The PEI-PAAIO/pDNA magnetoplex is highly water dispersible and suitable for long term storage, shows superparamagnetism, low cytotoxicity, high stability and nice transfection efficiency, and thus the PEI-PAAIO PEC can replace PEI as a non-viral gene vector.
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