The present invention provides a transmission device and transmission method for glass substrate. The transmission device includes a transmission channel, a calibration mechanism and a decompression mechanism; wherein the transmission channel for loading and transmitting the glass substrate, the calibration mechanism being for calibrating position of the glass substrate, and the decompression mechanism collaborating with calibrating mechanism for applying a force to the glass substrate reduce the friction between the transmission channel and the glass substrate when the calibration mechanism calibrating the position of the glass substrate. As such, the abrasion or damage problem to the glass substrate can be prevented so as to improve the yield rate of the glass substrate.