Invention Grant
- Patent Title: Compositions and methods to prevent AAV vector aggregation
- Patent Title (中): 防止AAV载体聚集的组成和方法
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Application No.: US12661553Application Date: 2010-03-19
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Publication No.: US09051542B2Publication Date: 2015-06-09
- Inventor: John Fraser Wright , Guang Qu
- Applicant: John Fraser Wright , Guang Qu
- Applicant Address: US MA Framingham
- Assignee: Genzyme Corporation
- Current Assignee: Genzyme Corporation
- Current Assignee Address: US MA Framingham
- Agency: Robins Law Group
- Agent Roberta L. Robins
- Main IPC: C12N7/00
- IPC: C12N7/00

Abstract:
Compositions and methods are provided for preparation of concentrated stock solutions of AAV virions without aggregation. Formulations for AAV preparation and storage are high ionic strength solutions (e.g. μ˜500 mM) that are nonetheless isotonic with the intended target tissue. This combination of high ionic strength and modest osmolarity is achieved using salts of high valency, such as sodium citrate. AAV stock solutions up to 6.4×1013 vg/mL are possible using the formulations of the invention, with no aggregation being observed even after ten freeze-thaw cycles. The surfactant Pluronic® F68 may be added at 0.001% to prevent losses of virions to surfaces during handling. Virion preparations can also be treated with nucleases to eliminate small nucleic acid strands on virions surfaces that exacerbate aggregation.
Public/Granted literature
- US20110076744A1 Compositions and methods to prevent AAV vector aggregation Public/Granted day:2011-03-31
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