Invention Grant
- Patent Title: Plasmid vector and transformant stably retaining plasmid
- Patent Title (中): 质粒载体和转化体稳定保留质粒
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Application No.: US11586574Application Date: 2006-10-26
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Publication No.: US09051589B2Publication Date: 2015-06-09
- Inventor: Shunsuke Sato
- Applicant: Shunsuke Sato
- Applicant Address: JP Osaka
- Assignee: KANEKA CORPORATION
- Current Assignee: KANEKA CORPORATION
- Current Assignee Address: JP Osaka
- Agency: Sughrue Mion, PLLC
- Priority: JP2005-312921 20051027
- Main IPC: C12P7/62
- IPC: C12P7/62 ; C12N15/74

Abstract:
The object of the present invention is to develop a novel vector. Preferably, the object is to develop a novel vector which can be stably retained in bacteria of the genus Ralstonia, Cupriavidus or Wautersia without any antibiotic-due selective pressure and has no transferability by conjugation. Another object is to provide a strain which can stably produce polyhydroxyalkanoate using the vector, and a method for producing a polyhydroxyalkanoate using the strain. The present invention provides a novel recombinant vector which contains an origin of DNA replication functioning in bacteria of the genus Ralstonia, Cupriavidus or Wautersia. Particularly, the transformant, which is obtained by using a recombinant vector which contains the origin of DNA replication functioning in bacteria of the genus Ralstonia, Cupriavidus or Wautersia and contains a region for a recombinant vector stabilization (par region) can make the vector to be stably retained in bacteria, and can efficiently produce a polyhydroxyalkanoate.
Public/Granted literature
- US20070099281A1 Novel plasmid vector and transformant stably retaining plasmid Public/Granted day:2007-05-03
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