Invention Grant
- Patent Title: RNA in situ hybridization
- Patent Title (中): RNA原位杂交
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Application No.: US12993338Application Date: 2009-05-19
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Publication No.: US09051610B2Publication Date: 2015-06-09
- Inventor: Hirofumi Doi , Masahiro Matsuoka , Tatsuhiro Nakano , Chizuru Nagayoshi
- Applicant: Hirofumi Doi , Masahiro Matsuoka , Tatsuhiro Nakano , Chizuru Nagayoshi
- Applicant Address: JP Chiba
- Assignee: CELISH FD, INC.
- Current Assignee: CELISH FD, INC.
- Current Assignee Address: JP Chiba
- Agency: Wenderoth, Lind & Ponack, L.L.P.
- Priority: JP2008-131302 20080519
- International Application: PCT/JP2009/059219 WO 20090519
- International Announcement: WO2009/142214 WO 20091126
- Main IPC: C12Q1/68
- IPC: C12Q1/68 ; G01N33/53 ; C12M1/34 ; G01N21/64 ; G01N31/22 ; C07H21/04 ; C07H21/02
Abstract:
A method for identifying the presence of a target gene mRNA is provided, which involves hybridizing one or more oligonucleic acid probes with the target gene mRNA expressed in a tissue sample, and detecting a low-molecular-weight compound label added to at least one of the bases of the oligonucleic acid probes. The oligonucleic acid probes are contacted with the tissue sample for hybridization with the target gene mRNA after pretreating (prehybridizing) one or more dummy oligonucleic acids with the tissue sample, or a mixture of the oligonucleic acid probes and the dummy oligonucleic acids is contacted with the sample tissue to hybridize the oligonucleic acid probes with the target gene mRNA.
Public/Granted literature
- US20110183331A1 RNA IN SITU HYBRIDIZATION Public/Granted day:2011-07-28
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