Invention Grant
US09074239B2 Flavin-binding glucose dehydrogenase, method for producing flavin-binding glucose dehydrogenase, and glucose measurement method
有权
黄素结合葡萄糖脱氢酶，黄素结合葡萄糖脱氢酶的生产方法和葡萄糖测定方法
- Patent Title: Flavin-binding glucose dehydrogenase, method for producing flavin-binding glucose dehydrogenase, and glucose measurement method
- Patent Title (中): 黄素结合葡萄糖脱氢酶，黄素结合葡萄糖脱氢酶的生产方法和葡萄糖测定方法
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Application No.: US14124559Application Date: 2012-06-06
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Publication No.: US09074239B2Publication Date: 2015-07-07
- Inventor: Ryoko Tajima , Kozo Hirokawa , Eriko Yoshihara , Yasuko Tanabe
- Applicant: Ryoko Tajima , Kozo Hirokawa , Eriko Yoshihara , Yasuko Tanabe
- Applicant Address: JP Noda-shi, Chiba
- Assignee: KIKKOMAN CORPORATION
- Current Assignee: KIKKOMAN CORPORATION
- Current Assignee Address: JP Noda-shi, Chiba
- Agency: Holtz, Holtz, Goodman & Chick PC
- Priority: JP2011-126893 20110607
- International Application: PCT/JP2012/064523 WO 20120606
- International Announcement: WO2012/169512 WO 20121213
- Main IPC: C12N9/04
- IPC: C12N9/04 ; C12Q1/54 ; C12Q1/00 ; C12Q1/32
Abstract:
A flavin-binding glucose dehydrogenase (FAD-GDH), which in addition to having high substrate specificity and adequate desirable heat stability, is suitable for efficient production, preferably using E. coli, yeast or molds and the like as host cells. The FAD-GDH has amino acid substitutions at positions equivalent to one or more locations selected from the group consisting of position 213, position 368 and position 526 in the amino acid sequence described in SEQ ID NO: 8. The FAD-GDH is acquired from a culture by inserting a gene encoding the FAD-GDH into host cells such as E. coli. A preferable example of the FAD-GDH is FAD-GDH, in which a signal peptide region present in an N-terminal region has been deleted from the amino acid sequence of Mucor-derived FAD-GDH, and which has the aforementioned amino acid substitutions. The FAD-GDH can be preferably used in clinical diagnosis.
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