There are provided an array substrate, a display device and a manufacturing method of the array substrate. The array substrate includes: a base substrate; a set of gate lines located over the base substrate; a black matrix that permeates into the base substrate around each of the gate lines. In technical solutions of the invention, the black matrix is located on the array substrate but not located on the color filter substrate, permeates into the base substrate around each gate line, and has a relatively high accuracy of alignment with the array substrate.