An optical fiber clamp and fabrication method thereof are disclosed. The optical fiber clamp includes one or more clamp units. Each clamp unit includes a clamp body formed of silicon, a guide hole formed under a top surface of the clamp body, the guide hole having an upper diameter greater than a lower diameter of the guide hole and having an inclined sidewall; and a locating hole connected to and extends downward from a bottom of the guide hole through the clamp body, the locating hole having an upper diameter equal to a lower diameter of the locating hole and smaller than the lower diameter of the guide hole.