A medical device for collecting cells includes a perforated tube and a brush disposed therein for collecting cells from tissue adjacent the tube. The tube includes a perforated portion and tissue can be drawn into the perforations in response to a vacuum applied to the tube. The brush can be inserted into the tube with the head portion of the brush aligned with the perforated portion to collect cells form the tissue that has been drawn into the perforations by the vacuum. The brush can be removed from the tube and another brush can be inserted to increase cell collection amounts.