Invention Grant
US09096878B2 Expression vector for producing protein derived from foreign gene in large quantity using animal cells, and use thereof
有权
用于使用动物细胞大量生产来自外来基因的蛋白质的表达载体及其用途
- Patent Title: Expression vector for producing protein derived from foreign gene in large quantity using animal cells, and use thereof
- Patent Title (中): 用于使用动物细胞大量生产来自外来基因的蛋白质的表达载体及其用途
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Application No.: US13140905Application Date: 2009-12-22
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Publication No.: US09096878B2Publication Date: 2015-08-04
- Inventor: Hiroshi Tahara , Yusuke Suzuki , Keiichi Yamamoto , Yuzuru Kitahara , Yasuhiko Suzuki
- Applicant: Hiroshi Tahara , Yusuke Suzuki , Keiichi Yamamoto , Yuzuru Kitahara , Yasuhiko Suzuki
- Applicant Address: JP Hokkaido JP Osaka
- Assignee: National University Corporation Hokkaido University,Fuso Pharmaceutical Industries, Ltd.
- Current Assignee: National University Corporation Hokkaido University,Fuso Pharmaceutical Industries, Ltd.
- Current Assignee Address: JP Hokkaido JP Osaka
- Agency: Bozicevic, Field & Francis LLP
- Agent Karl Bozicevic
- Priority: JP2008-325690 20081222
- International Application: PCT/JP2009/071326 WO 20091222
- International Announcement: WO2010/074080 WO 20100701
- Main IPC: C12P21/02
- IPC: C12P21/02 ; C12N15/85
Abstract:
The present inventors conducted dedicated studies and successfully constructed expression vectors that enable high-level production of foreign gene-derived proteins in mammalian host cells, which comprise a translation-impaired dihydrofolate reductase gene cistron whose expression has been attenuated by altering the codons to the least frequently used codons in mammals; and a gene cassette which has a cloning site for incorporation of a foreign gene between a highly transcriptionally active promoter and a highly stable polyadenylation signal.
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