Invention Grant
US09096897B2 Compositions and methods for quantifying a nucleic acid sequence in a sample comprising a primer oligonucleotide with a 3′-terminal region comprising a 2′-modified nucleotide
有权
用于定量包含引物寡核苷酸的样品中的核酸序列的组合物和方法，所述引物寡核苷酸具有包含2'-修饰的核苷酸的3'末端区域
- Patent Title: Compositions and methods for quantifying a nucleic acid sequence in a sample comprising a primer oligonucleotide with a 3′-terminal region comprising a 2′-modified nucleotide
- Patent Title (中): 用于定量包含引物寡核苷酸的样品中的核酸序列的组合物和方法，所述引物寡核苷酸具有包含2'-修饰的核苷酸的3'末端区域
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Application No.: US14342766Application Date: 2013-04-09
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Publication No.: US09096897B2Publication Date: 2015-08-04
- Inventor: Daniel Shaffer , Stephen A. Judice
- Applicant: ENVIROLOGIX, INC.
- Applicant Address: US ME Portland
- Assignee: EnviroLogix Inc.
- Current Assignee: EnviroLogix Inc.
- Current Assignee Address: US ME Portland
- Agency: Saul Ewing LLP
- Agent Melissa Hunter-Ensor; Elbert C. Chiang
- International Application: PCT/US2013/035750 WO 20130409
- International Announcement: WO2013/155056 WO 20131017
- Main IPC: C12P19/34
- IPC: C12P19/34 ; C12Q1/68
Abstract:
The present invention features compositions and methods for quantifying detection of a target oligonucleotide in a sample in real time comprising one or more primer oligonucleotides comprising a 5′ nicking enzyme recognition site and a 3′-terminal region comprising a 2′-modified nucleotide. These methods are compatible with target oligonucleotides amplified using a nicking amplification reaction.
Public/Granted literature
- US20150104788A1 COMPOSITIONS AND METHODS QUANTIFYING A NUCLEIC ACID SEQUENCE IN A SAMPLE Public/Granted day:2015-04-16
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