A specimen slicing guide is provided for assisting in slicing a specimen taken from a human or an animal. The specimen slicing guide includes a base extending in a direction having a horizontal component. A plurality of needles extend from the base with gaps provided between the needles in the extension direction of the base. The needles extend at least substantially parallel to each other and at least substantially perpendicular to the horizontal component of base. A needle spacer has a plurality of holes and the plurality of needles are respectively disposed in the plurality of holes. The needle spacer is configured to press against the specimen and to move relative to the base.