Invention Grant
- Patent Title: DNA polymerases and related methods
- Patent Title (中): DNA聚合酶及相关方法
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Application No.: US12425303Application Date: 2009-04-16
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Publication No.: US09102924B2Publication Date: 2015-08-11
- Inventor: Keith A. Bauer , Ellen Fiss , David H. Gelfand , Edward S. Smith , Shawn Suko , Olga Budker , Nancy Schoenbrunner , Susanne Stoffel , Thomas Myers
- Applicant: Keith A. Bauer , Ellen Fiss , David H. Gelfand , Edward S. Smith , Shawn Suko , Olga Budker , Nancy Schoenbrunner , Susanne Stoffel , Thomas Myers
- Applicant Address: US CA Pleasanton
- Assignee: Roche Molecular Systems, Inc.
- Current Assignee: Roche Molecular Systems, Inc.
- Current Assignee Address: US CA Pleasanton
- Agency: Kilpatrick Townsend & Stockton LLP
- Main IPC: C12N9/12
- IPC: C12N9/12

Abstract:
Disclosed are mutant DNA polymerases having improved extension rates relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. The mutant polymerases overcome the inhibitory effects by an intercalating dye. Therefore, the mutant polymerases are useful in a variety of disclosed methods in combination with an intercalating dye. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases.
Public/Granted literature
- US20090280539A1 DNA POLYMERASES AND RELATED METHODS Public/Granted day:2009-11-12
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