Invention Grant
US09120871B2 Process for preparing FGF21 with low degree of O-glycosylation
有权
制备具有低O-糖基化程度的FGF21的方法
- Patent Title: Process for preparing FGF21 with low degree of O-glycosylation
- Patent Title (中): 制备具有低O-糖基化程度的FGF21的方法
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Application No.: US13522804Application Date: 2011-01-20
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Publication No.: US09120871B2Publication Date: 2015-09-01
- Inventor: Rita Slaaby , Inger Lautrup-Larsen
- Applicant: Rita Slaaby , Inger Lautrup-Larsen
- Applicant Address: DK Bagsvaerd
- Assignee: Novo Nordisk A/S
- Current Assignee: Novo Nordisk A/S
- Current Assignee Address: DK Bagsvaerd
- Agent Nonna G. Akopyan
- Priority: EP09151227 20090123; EP09152144 20090205; EP09164904 20090708; WOPCT/EP2010/050720 20100122; EP10165927 20100615
- International Application: PCT/EP2011/050718 WO 20110120
- International Announcement: WO2011/089170 WO 20110728
- Main IPC: C12N1/19
- IPC: C12N1/19 ; C07K14/50 ; A61K38/00
Abstract:
Expression of FGF21 in a S. cerevisiae pmt2 knock out strain reduces the O-glycosylation significantly and does not decrease the expression level. Expression in mkc7 knock out strain can decrease degradation of FGF21 and analogues thereof. Point mutations at position R17 and R36 can decrease degradation of FGF21 and analogues thereof when they are expressed in yeast.
Public/Granted literature
- US20130005951A1 PROCESS FOR PREPARING FGF21 WITH LOW DEGREE OF O-GLYCOSYLATION Public/Granted day:2013-01-03
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