Invention Grant
- Patent Title: Method for inducing differentiation of human pluripotent stem cell into intermediate mesoderm cell
- Patent Title (中): 诱导人多能干细胞分化为中胚层细胞的方法
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Application No.: US13811163Application Date: 2011-07-21
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Publication No.: US09121011B2Publication Date: 2015-09-01
- Inventor: Kenji Osafune , Shin-ichi Mae , Masatoshi Kajiwara
- Applicant: Kenji Osafune , Shin-ichi Mae , Masatoshi Kajiwara
- Applicant Address: JP Kyoto-shi
- Assignee: KYOTO UNIVERSITY
- Current Assignee: KYOTO UNIVERSITY
- Current Assignee Address: JP Kyoto-shi
- Agency: Birch, Stewart, Kolasch & Birch, LLP
- International Application: PCT/JP2011/067181 WO 20110721
- International Announcement: WO2012/011610 WO 20120126
- Main IPC: C12N5/071
- IPC: C12N5/071 ; C12N5/074 ; C12N5/073 ; C12N5/0735 ; G01N33/50

Abstract:
The present invention relates to: a method for producing an intermediate mesoderm cell from a human pluripotent stem cell, comprising a step of culturing the human pluripotent stem cell in a medium containing Activin A and Wnt or a functional equivalent of Wnt and a step of culturing cells in a medium containing BMP and Wnt or a functional equivalent of Wnt; to a method for producing a metanephric cell from the intermediate mesoderm cell produced by the first method; to a human pluripotent stem cell having a foreign reporter gene in the chromosome wherein the gene is expressed interlocked with the expression of endogenous OSR1; to a method for screening for an inducer for differentiation into intermediate mesoderm using the human pluripotent stem cell; and to a kit for inducing the differentiation into an intermediate mesoderm cell.
Public/Granted literature
- US20130122536A1 METHOD FOR INDUCING DIFFERENTIATION OF HUMAN PLURIPOTENT STEM CELL INTO INTERMEDIATE MESODERM CELL Public/Granted day:2013-05-16
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