Invention Grant
- Patent Title: Polymerase chain reaction product-cloning vector suitable to its easy production and method for producing the same
- Patent Title (中): 聚合酶链反应产物克隆载体适合其易于生产及其生产方法
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Application No.: US12740003Application Date: 2008-10-29
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Publication No.: US09121023B2Publication Date: 2015-09-01
- Inventor: Seongjun Yoon , SooYoun Jun , SangHyeon Kang
- Applicant: Seongjun Yoon , SooYoun Jun , SangHyeon Kang
- Applicant Address: KR Sungnam-Si
- Assignee: INTRON BIOTECHNOLOGY, INC.
- Current Assignee: INTRON BIOTECHNOLOGY, INC.
- Current Assignee Address: KR Sungnam-Si
- Agency: Ballard Spahr LLP
- Priority: KR10-2007-0109265 20071030
- International Application: PCT/KR2008/006378 WO 20081029
- International Announcement: WO2009/057944 WO 20090507
- Main IPC: C12N15/00
- IPC: C12N15/00 ; C12N15/64 ; C12N15/70
Abstract:
The present invention relates to a PCR product cloning vector applicable directly to the cloning of a PCR product. More precisely, the present invention relates to a PCR product cloning vector designed to be effective in use for blue/white colony selection and produced based on the restriction enzyme treatment process to generate non-complementary unpaired single overhang and a method for producing the same. According to the method of the present invention, the PCR product cloning vector designed to be advantageous for blue/white cloning selection can be produced with improved efficiency compared with the convention method.
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