Invention Grant
- Patent Title: Reducing template independent primer extension and threshold time for loop mediated isothermal amplification
- Patent Title (中): 减少模板独立的引物延伸和循环介导的等温扩增的阈值时间
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Application No.: US13671123Application Date: 2012-11-07
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Publication No.: US09121046B2Publication Date: 2015-09-01
- Inventor: Nathan Tanner , Thomas C. Evans, Jr.
- Applicant: New England Biolabs, Inc.
- Applicant Address: US MA Ipswich
- Assignee: New England Biolabs, Inc.
- Current Assignee: New England Biolabs, Inc.
- Current Assignee Address: US MA Ipswich
- Agency: New England Biolabs, Inc
- Agent Harriet M. Strimpel
- Main IPC: C12P19/34
- IPC: C12P19/34 ; C12Q1/68
Abstract:
Compositions and methods are provided for loop mediated isothermal amplification in which single stranded binding proteins are shown to protect primers from non-specific extension and to stimulate the rate of threshold amplification.
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