Invention Grant
- Patent Title: Methods and compositions for discrimination between cytosine and modifications thereof and for methylome analysis
- Patent Title (中): 用于鉴别胞嘧啶及其修饰物和甲基化分析的方法和组合物
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Application No.: US13827885Application Date: 2013-03-14
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Publication No.: US09121061B2Publication Date: 2015-09-01
- Inventor: Romualdas Vaisvila , Heidi Erika Johnson , Saulius Vainauskas , Theodore B. Davis
- Applicant: New England Biolabs, Inc.
- Applicant Address: US MA Ipswich
- Assignee: New England Biolabs, Inc.
- Current Assignee: New England Biolabs, Inc.
- Current Assignee Address: US MA Ipswich
- Agency: New England Biolabs, Inc
- Agent Harriet M. Strimpel
- Main IPC: C12Q1/68
- IPC: C12Q1/68
Abstract:
Compositions and methods are provided for discrimination between cytosine and modifications thereof using cytidine deaminases and/or oxygenases. Variants of wild type cytidine deaminases are described which show reduced bias with respect to adjacent nucleotides upstream of the cytosine. The methods provide a rapid and convenient use of enzymes to obtain methylomes.
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