A multi-core optical fiber has: a plurality of core portions; a cladding portion that is positioned around each of the plurality of core portions and has a refractive index lower than that of each of the plurality of core portions; and a separation distance between adjacent ones of the plurality of core portions being set so that crosstalk of light between the adjacent core portions over an entire length thereof becomes −15 dB or greater at a wavelength of 1550 nm and a cable cut-off wavelength becomes 1530 nm or less.