A method of loading a locked tissue biopsy needle into a biopsy gun includes rotating a head of the biopsy gun in a first direction, with the head located at a front distal portion of the biopsy gun. The method includes inserting the locked tissue biopsy needle into a distal end of the head of the biopsy gun. The locked tissue biopsy needle provides a stylet inserted in a longitudinal lumen of a cannula, and the stylet is locked in position relative to the cannula thus preventing longitudinal movement of the stylet within the cannula. The method includes rotating the head of the biopsy gun in a second direction opposite from the first direction and rotating the stylet relative to the cannula and unlocking the stylet from the cannula allowing longitudinal movement of the stylet within the cannula.