Disclosed is an anti-reflection film comprising a high refractive index layer comprising a first binder including a crosslinked polymer of a multifunctional (meth)acrylate compound, and nano silica particles dispersed in the first binder; and a low refractive index layer laminated on the high refractive index layer, and comprising a second binder including a crosslinked copolymer of a multifunctional (meth)acrylate compound and a fluorine-based (meth)acrylate compound, and hollow silica particles and hollow silica particles coated with a fluorine-based compound on the surface, which are dispersed in the second binder, wherein the weight ratio of the hollow silica particles to the hollow silica particles coated with a fluorine-based compound is 1:1˜10. Also disclosed is a method for manufacturing the anti-reflection film using a composition that is separated into at least two layers by phase separation after single layer coating by a more simplified process.