Invention Grant
US09127307B2 Reagents and methods relating to DNA assays using amplicon probes on encoded particles
有权
使用扩增子探针对编码的颗粒进行DNA测定的试剂和方法
- Patent Title: Reagents and methods relating to DNA assays using amplicon probes on encoded particles
- Patent Title (中): 使用扩增子探针对编码的颗粒进行DNA测定的试剂和方法
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Application No.: US13773089Application Date: 2013-02-21
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Publication No.: US09127307B2Publication Date: 2015-09-08
- Inventor: Karl Edwin Adler, Jr. , Mack J. Schermer
- Applicant: PerkinElmer Health Sciences, Inc.
- Applicant Address: US MA Waltham
- Assignee: PerkinElmer Health Sciences, Inc.
- Current Assignee: PerkinElmer Health Sciences, Inc.
- Current Assignee Address: US MA Waltham
- Agency: Gifford, Krass, Sprinkle, Anderson & Citkowski, P.C.
- Main IPC: C12P19/34
- IPC: C12P19/34 ; C12Q1/68
Abstract:
Encoded bead multiplex assays for chromosomal gains and losses are provided that provide the benefits of complex, large template DNA sources, such as BAC DNA, as the probe material without bead networking or other assay performance problems. Reagents for assaying DNA are described herein which include a plurality of encoded particles having attached amplicons amplified from a template DNA sequence. Each individual attached amplicon includes a nucleic acid sequence identical to a random portion of the template DNA sequence, wherein the amplicons together represent substantially the entire template DNA and wherein the nucleic acid sequence identical to a random portion of the template DNA sequence of each individual amplicon is shorter than the entire template DNA.
Public/Granted literature
- US20130157899A1 REAGENTS AND METHODS RELATING TO DNA ASSAYS USING AMPLICON PROBES ON ENCODED PARTICLES Public/Granted day:2013-06-20
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