A remote water shut-off valve system for isolating a water supply line to a structure includes the structure having a vertical wall with a shut-off valve fluidly located in a water supply line for sealing an upstream side of the water supply line from a downstream side to isolate water flow. The system includes a microprocessor, a valve receiver, a valve actuator, an activation switch, and a remote transmitter. For operation, an activation signal is sent to the microprocessor via the remote transmitter or the activation switch. The activation signal is sent to the actuator from the microprocessor. The actuator moves the shut-off valve into either an open or a closed position. The shut-off valve divides an upstream side of the water supply line from a downstream side of the water supply line to isolate water flow.