Invention Grant
- Patent Title: Up-concentration of organic microobjects for microscopic imaging
- Patent Title (中): 用于显微镜成像的有机微量物质的上调
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Application No.: US13263997Application Date: 2010-04-14
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Publication No.: US09128016B2Publication Date: 2015-09-08
- Inventor: Bas Hulsken , Stein Kuiper , Sjoerd Stallinga , Bo Joakim Isaksson , Mark W. G. Ponjee , Bart E. G. J. Van Meerbergen , Zeynep S. Unay
- Applicant: Bas Hulsken , Stein Kuiper , Sjoerd Stallinga , Bo Joakim Isaksson , Mark W. G. Ponjee , Bart E. G. J. Van Meerbergen , Zeynep S. Unay
- Applicant Address: NL Eindhoven
- Assignee: Koninklijke Philips N.V.
- Current Assignee: Koninklijke Philips N.V.
- Current Assignee Address: NL Eindhoven
- Priority: EP09305317 20090414
- International Application: PCT/IB2010/051617 WO 20100414
- International Announcement: WO2010/119408 WO 20101021
- Main IPC: G01N15/06
- IPC: G01N15/06 ; G01N1/40

Abstract:
A method of analyzing a sample fluid containing organic microobjects is proposed. The method comprises the steps of: up-concentrating (S1) the microobjects by removing, in a total time T1, a volume V1 of the sample fluid from the upconcentrate sample microobjects; immersing (S2) the microobjects in a transfer fluid, or leaving the microobjects in a remaining portion of the sample fluid, the remaining portion of the sample fluid then providing the transfer fluid; filtering (S3), in a total time T3, a volume V3 of the transfer fluid by a filter, thereby accumulating the microobjects on the filter; and generating (S4) an image of the microobjects accumulated on the filter; wherein the throughput V1/T1 of the step of up-concentrating (S1) is greater than the throughput V1/T1, of the step of filtering (S3). The filter may be a second filter, and the step of up-concentrating (S1) may involve: filtering the sample fluid by a first filter, thereby accumulating the microobjects on the first filter. An apparatus or system for analyzing a sample fluid containing organic microobjects is also disclosed.
Public/Granted literature
- US20120034623A1 UP-CONCENTRATION OF ORGANIZ MICROOBJECTS FOR MICROSCOPIC IMAGING Public/Granted day:2012-02-09
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