Invention Grant
US09128090B2 Rapid way to obtain high expression clones of mammalian cells using a methylcellulose and immunoprecipitation screening method
有权
使用甲基纤维素和免疫沉淀筛选方法快速获得哺乳动物细胞高表达克隆
- Patent Title: Rapid way to obtain high expression clones of mammalian cells using a methylcellulose and immunoprecipitation screening method
- Patent Title (中): 使用甲基纤维素和免疫沉淀筛选方法快速获得哺乳动物细胞高表达克隆
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Application No.: US10928815Application Date: 2004-08-27
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Publication No.: US09128090B2Publication Date: 2015-09-08
- Inventor: Chichang Lee , Celia Ly , Gordon Moore
- Applicant: Chichang Lee , Celia Ly , Gordon Moore
- Applicant Address: US PA Horsham
- Assignee: Janssen Biotech, Inc.
- Current Assignee: Janssen Biotech, Inc.
- Current Assignee Address: US PA Horsham
- Agent Brian C. Carey
- Main IPC: C12Q1/68
- IPC: C12Q1/68 ; G01N21/82 ; C07K16/00 ; G01N33/569 ; C07K16/24
Abstract:
The invention provides a genetic screening method for identifying a transfected cell expressing the polypeptide of interest. The methods allows for high throughput screening of recombinant cells for elevated levels of expression of the polypeptide of interest. The invention also provides capture media, formulations and methods of making and using thereof.
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