A multi-core optical fiber includes a plurality of core portions, and a cladding portion positioned at outer peripheries of the plurality of core portions, the cladding portion having a refractive index lower than a maximum refractive index of each of the core portions, in which each of the core portions propagates light only with predetermined number, which is equal to or greater than 2, of propagation modes, and an effective core area at wavelength of 1550 nm of each of the propagation modes is equal to or greater than 120 μm2.