A display apparatus and an operation method thereof are provided. The display apparatus includes a display panel. The display panel includes a plurality of data lines, a plurality of scan lines and a plurality of pixels. The pixels are arranged in a matrix manner, and each pixel is electrically connected to one of the data lines and one of the scan lines. The operation method includes steps of: dividing the scan lines into N scan line groups, wherein N is an integer from 2 to the number of the scan lines; and in N frame periods sequentially driving the N scan line groups of scan line respectively and thereby sequentially updating display data of the pixels electrically connected to the N scan line groups of the scan line respectively.