An anti-counterfeiting system and method for an e-ticket are disclosed. The anti-counterfeiting system includes a ticket service center, a handheld device, a ticket checking device, and a database. The ticket checking device identifies the e-ticket information shown on a display of the handheld device at least twice. The e-ticket is verified by using the handheld device to execute a ticket software including an e-ticket service. The handheld device obtains at least one e-ticket information from the ticket service center via a network link. When the display of the handheld device shows that the e-ticket information is read by the ticket checking device and is verified by the database, the ticket service center transmits an e-ticket verification information to the handheld device, and the display of the handheld device then shows the e-ticket information for the ticket checking device to check again.