A device and method for treatment of hemorrhoids, in which the devices includes: a housing insertable into the rectum; a tissue port configured to receive a tissue segment containing at least a portion of a hemorrhoidal blood vessel; opposing needle chambers including at least one pair of corresponding needles coupled by a suture in which the pair of corresponding needles are substantially opposite to one another and in an active position substantially aligned with the tissue segment; a needle driver that advances the pair of corresponding needles through the tissue segment to encircle a portion of the hemorrhoidal blood vessel with the suture, thereby ligating the hemorrhoidal blood vessel; and an actuator coupled to at least one of the needle chambers that actuates the needle chambers such that another pair of corresponding needles is in the active position.