A system and method for disinfecting an exposed portion of a female luer connector is disclosed. A male luer connector coupled to a male luer connector cap is provided where the male luer connector cap has a chamber containing a disinfectant and a sealing member for sealing the disinfectant in the chamber. The chamber is at least partly opened and the disinfectant exposed by movement of the sealing member. An exposed surface of a female luer connector is caused to come in contact with the disinfecting fluid in the chamber prior to the female luer connector mating with the male luer connector.