Invention Grant
US09150897B2 Expression and purification of fusion protein with multiple MBP tags
有权
具有多个MBP标签的融合蛋白的表达和纯化
- Patent Title: Expression and purification of fusion protein with multiple MBP tags
- Patent Title (中): 具有多个MBP标签的融合蛋白的表达和纯化
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Application No.: US13814713Application Date: 2011-08-05
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Publication No.: US09150897B2Publication Date: 2015-10-06
- Inventor: Stephen Kowalczykowski , Ryan Jensen
- Applicant: Stephen Kowalczykowski , Ryan Jensen
- Applicant Address: US CA Oakland
- Assignee: The Regents of the University of California
- Current Assignee: The Regents of the University of California
- Current Assignee Address: US CA Oakland
- Agency: Kilpatrick Townsend & Stockton LLP
- International Application: PCT/US2011/046846 WO 20110805
- International Announcement: WO2012/019157 WO 20120209
- Main IPC: C12P21/06
- IPC: C12P21/06 ; C12N1/20 ; C07K1/00 ; C12P21/00 ; C12N15/62
Abstract:
The present invention provides a new method for recombinantly expressing a protein of interest, such as the human BRCA2 protein, BLM protein, CtIP protein, or EXOI protein, by expressing the protein in the form of a fusion protein comprising two maltose-binding protein (MBP) or glutathione-S-transferase (GST) tags. The expression cassette useful for this method and the fusion protein produced by this method are also described.
Public/Granted literature
- US20130203116A1 EXPRESSION AND PURIFICATION OF FUSION PROTEIN WITH MULTIPLE MBP TAGS Public/Granted day:2013-08-08
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