Invention Grant
- Patent Title: Coincidence detection
- Patent Title (中): 一致性检测
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Application No.: US13806050Application Date: 2011-06-23
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Publication No.: US09150904B2Publication Date: 2015-10-06
- Inventor: Peter Parker , Michela Perani
- Applicant: Peter Parker , Michela Perani
- Applicant Address: GB London
- Assignee: King's College London
- Current Assignee: King's College London
- Current Assignee Address: GB London
- Agency: Bradley Arant Boult Cummings LLP
- Agent Thomas G. Peterson
- Priority: GB1010598.9 20100623
- International Application: PCT/GB2011/000953 WO 20110623
- International Announcement: WO2011/161420 WO 20111229
- Main IPC: C12Q1/68
- IPC: C12Q1/68 ; G01N33/53 ; C12N15/10 ; C12N15/62 ; G01N33/68

Abstract:
The invention relates to a method of detecting the coincidence of two biomolecular structures in a solid phase sample, said method comprising: (i) providing a first and a second fusion protein, each fusion protein comprising (a) a detection domain, said detection domain comprising a DNA binding domain; said detection domain capable of binding a cognate specific nucleotide sequence in co-operation with a further detection domain; (b) a recognition domain, said recognition domain capable of binding a target biomolecular structure; and (c) a connector domain; said connector domain being fused at one end to the detection domain and being fused at the other end to the recognition domain; wherein at least two of (a), (b) and (c) are heterologous to one another; wherein the recognition domains of said first and said second fusion proteins are capable of binding to first and second biomolecular structures; (ii) contacting the sample with said first and second fusion proteins; (iii) incubating to allow binding; (iv) removing unbound fusion protein; (v) contacting the sample with nucleic acid comprising said cognate specific nucleotide sequence; (vi) incubating to allow heterotrimeric binding of the nucleic acid; (vii) detecting nucleic acid bound to the sample wherein detection of nucleic acid in step; (vii) indicates that the two biomolecular structures are present coincidentally in said sample.
Public/Granted literature
- US20130171644A1 COINCIDENCE DETECTION Public/Granted day:2013-07-04
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